Deletion doesn’t influence mEPSC peak amplitude in DG granule cells. a Instance Stromelysin-1/MMP-3 Protein C-6His traces of NMDAR/ AMPAR (N/A) ratio recordings three weeks following injection of AAV-Cre-T2AGFP. b N/A ratio is strongly lowered 3 weeks just after NMDAR deletion (GluN1-/-) in comparison to cells injected having a manage virus (AAV-T2AtdTom = GluN1fl/fl). c-h CT100(I716F) overexpression does not influence peak amplitude (blue bars). Peak amplitude is improved in GluN2B-/- when compared with GluN2B-/-/CT100(I716F) DG granule cells. Bar graphs show median IQR. * = p 0.05, ** = p 0.01, *** = p 0.001, norm. = normalized, cum. = cumulative, ampl. = amplitude (PDF 1391 kb) Added file 3: S2. Synaptic depression induced by CT100 overexpression is NMDAR dependent in young mice. a Example traces of mEPSC recordings from mice injected with AAV-Tom (GluN1fl/fl), AAV-CT100-T2A-Tom (GluN1fl/fl/ CT100), AAV-Cre-T2A-GFP (GluN1-/-) or co-injected with AAV-CT100-T2A-Tom and AAV-Cre-T2A-GFP (GluN1-/-/CT100). b Cumulative probability of inter-eventinterval (IEI) is shifted to longer IEIs in CT100(I716F) overexpressing cells. c mEPSC frequency is decreased in CT100-overexpressing and enhanced in GluN1-/- DG granule cells. There isn’t any difference between GluN1-/- cells and GluN1-/-/CT100 DG granule cells. e f Peak amplitude is elevated in GluN1-/- cells in comparison with GluN1fl/fl cells. Cumulative probability of your amplitude is shifted towards larger amplitues in GluN1-/- neuons. d CT100 increased the spine quantity of DG granule cells from slices of young mice. g The quantification with the spine morphology distribution shows no considerable difference in between the groups. Bar graphs show median IQR. * = p 0.05, ** = p 0.01, *** = p 0.001; cum. = cumulative; morph. = morphology (PDF 1485 kb) More file 4: S3. Active and passive properties of DG granule cells usually are not altered by CT100(I716F) overexpression. a Instance traces of action potentials (APs) from manage and CT100(I716F)-overexpressing DG granule cells. b CT100(I716F) overexpression will not alter the intrinsic properties threshold, amplitude, half-amplitude (HA) duration, afterhyperpolarization (AHP) and input resistance of DG granule cells compared to control cells. c Firing frequency, earlyand late adaptation do not differ in between handle and CT100(I716F)-overexpressing DG granule cells. d Instance traces of firing patterns of handle and CT100(I716F) DG granule cells. Bar graphs show median IQR. (PDF 146 kb) Further file five: S5. CT100(I716F) overexpression will not influence total dendritic length in adult mice. a Examples of traced DG granule cells of the GluN2Afl/fl mouse line. b The number of intersections analyzed by Sholl analysis is not changed by CT100(I716F) overexpression, GluN2A subunit deletion and GluN2A deletion in mixture with CT100(I716F) overexpression. Imply SEM. Total dendritic length is just not distinct between the groups. c Examples of traced DG granule cells of the GluN2Bfl/fl mouse line. d Sholl analysis of the number of intersections shows subtle modifications in dendritic complexity in GluN2B-/ – /CT100(I716F) cells in comparison to their respective handle (GluN2B-/-). Mean SEM. Total dendritic length is just not unique in between the groups. Bar graphs show median IQR.; dendr. = dendritic, morph. = morphology (PDF 133 kb) Further file six: S6. Functional and structural properties aren’t impacted in six-month old 5xFAD mice. a Examples of traced DG granule cells of sixmonth old WT and 5xFAD mice. b The number.
