Product Name: GLS2 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: GLS2 antibody can be used for detection of GLS2 by Western blot at 0.5 – 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 36, 55, 62, 66 kDa Observed: 55, 62 kDa
Immunogen: GLS2 antibody was raised against an 18 amino acid synthetic peptide near the center terminus of human GLS2.The immunogen is located within amino acids 300 – 350 of GLS2.
Host Species: Rabbit
Purification: GLS2 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1437321-24-8
HG-9-91-01
Buffer: GLS2 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: GLS2 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: GLS2 Antibody: GA, GLS, LGA, hLGA, GA, Glutaminase liver isoform, mitochondrial, L-glutaminase
Accession NO.: NP_037399
Protein Ino: 20336214
Official Symbol: GLS2
Geneid: 27165
Background: GLS2 Antibody: Phosphate-activated glutaminase, also known as Glutaminase 2 (GLS2), was initially isolated from rat liver, although it has been shown to be expressed in other tissues. Like the functionally similar, larger kidney glutaminase, GLS2 catalyzes the hydrolysis of glutamine to stoichiometric amounts of glutamate and ammonia. Expression of GLS2 is increased by p53 under both stressed and nonstressed conditions, resulting in increased levels of glutamate and alpha-ketoglutarate, which in turn results in enhanced mitochondrial respiration and ATP generation. GLS2 also regulates antioxidant defense function in cells by increasing reduced glutathione levels and decreasing ROS-levels, suggesting that GLS2 acts as a mediator of p53s role in antioxidant defense in addition to its role in energy metabolism.
PubMed ID:http://aac.asm.org/content/18/3/416.abstract
