Product Name: DCLK1 Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: ELISA, IF, IHC-P, WB
Applications: DCLK1 antibody can be used for detection of DCLK1 by Western blot at 0.5 – 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: Predicted: 90 kDa Observed: 85 kDa
Immunogen: DCLK1 antibody was raised against a 14 amino acid synthetic peptide near the amino terminus of human DCLK1.The immunogen is located within the last 50 amino acids of DCLK1.
Host Species: Rabbit
Purification: DCLK1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1019206-88-2
Product: Regorafenib (monohydrate)
Buffer: DCLK1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: DCLK1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: DCLK1 Antibody: CL1, DCLK, CLICK1, DCDC3A, DCAMKL1, KIAA0369, Doublecortin domain-containing protein 3A
Accession NO.: NP_004725
Protein Ino: 4758128
Official Symbol: DCLK1
Geneid: 9201
Background: DCLK1 Antibody: DCLK1 is one of three doublecortin-like kinases similar to the Ca2+/calmodulin-dependent protein kinase (CaMK) family. DCLK1 mRNA, like that of the homologous DCLK2 and DCLK3, is highly expressed in adult brain, but only DCLK1 and DCLK2 transcripts are present in human fetal brain and the developing mouse embryo, suggesting that DCLK1 and DCLK2 may play roles in cortical development. The DCLK proteins are homologous to Doublecortin (DCX), a gene that is mutated in X-linked human lissencephaly. In mouse models where the DCX gene has been disrupted, DCLK1 expression increases slightly and appears to compensate for the loss of DCX, as mice mutant for both DCX and DCLK1 show a severe phenotype including perinatal lethality, disorganized neocortical layering, and profound hippocampal cytoarchitectural disorganization. Unlike DCLK1, DCLK2 expression does not change in DCX-null mice.
PubMed ID:http://aac.asm.org/content/38/3/628.abstract
