Product Name: Chk2 (phospho Thr383) Antibody
Species Reactivity: Human, Mouse, Rat
Tested Applications: IF, WB
Applications: Western Blot: 1:500~1:1000, Immunofluorescence: 1:100~1:200
User Note:
Predicted Molecular Weight: 60 kDa
Immunogen: Chk2 (phospho Thr383) antibody was raised against a peptide sequence around phosphorylation site of threonine383 (M-R-T (p)-L-C) derived from Human Chk1.
Host Species: Rabbit
Purification: Antibodies were purified by affinity-chromatography using epitope-specific peptide.
Physical State: Liquid
CAS NO.: 22033-87-0
Product: TRO 19622
Buffer: Antibody supplied in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration: 1 mg/mL
Storage Conditions: Store antibody at -20˚C for up to one year.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CHEK2, CHK2, Cds1, Chk2, RAD53
Accession NO.: NP_009125.1
Protein Ino: 6005850
Official Symbol: CHEK2
Geneid: 11200
Background: In response to DNA damage and replication blocks, cell cycle progression is halted through the control of critical cell cycle regulators. The protein encoded by Chk2 gene is a cell cycle checkpoint regulator and putative tumor suppressor. It contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. When activated, the encoded protein is known to inhibit CDC25C phosphatase, preventing entry into mitosis, and has been shown to stabilize the tumor suppressor protein p53, leading to cell cycle arrest in G1. In addition, this protein interacts with and phosphorylates BRCA1, allowing BRCA1 to restore survival after DNA damage. Mutations in this gene have been linked with Li-Fraumeni syndrome, a highly penetrant familial cancer phenotype usually associated with inherited mutations in TP53. Also, mutations in this gene are thought to confer a predisposition to sarcomas, breast cancer, and brain tumors. This nuclear protein is a member of the CDS1 subfamily of serine/threonine protein kinases. Three transcript variants encoding different isoforms have been found for this gene.
PubMed ID:http://aac.asm.org/content/36/1/163.abstract
