Product Name: Calponin Antibody [CLPN5-1]
Species Reactivity: Human, Rat
Tested Applications: IF, IHC-P
Applications: Immunofluorescence: 1-2 ug/mlImmunohistochemistry (FFPE): 0.5-1 ug/ml for 30 min at RT (1)Prediluted format : incubate for 30 min at RT (2)Optimal dilution of the Calponin antibody should be determined by the researcher.1. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 min2. The prediluted format is supplied in a dropper bottle and is optimized for use in IHC. After epitope retrieval step (if required), drip mAb solution onto the tissue section and incubate at RT for 30 min.
User Note: Optimal dilutions for each application to be determined by the researcher
Predicted Molecular Weight:
Immunogen: Recombinant human protein was used as the immunogen for the Calponin antibody.
Host Species: Mouse
Purification: Protein G affinity chromatography
Physical State: Liquid
CAS NO.: 59-30-3
Product: Folic acid
Buffer: PBS with 0.1 mg/ml BSA and 0.05% sodium azide
Concentration: 0.2 mg/mL
Storage Conditions: Aliquot and Store at -20C. Avoid freez-thaw cycles.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: CNN1, Basic calponin, Calponin, Calponin-1, Calponins, basic, Calponin H1, smooth muscle, SMCC, Sm-Calp
Accession NO.:
Protein Ino:
Official Symbol: CNN1
Geneid: 1264
Background: Calponin is a calmodulin, F-actin and tropomyosin binding protein which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle.
PubMed ID:http://aac.asm.org/content/43/2/354.abstract
