Product Name: CDK7 Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: CDK7 antibody can be used for detection of CDK7 by ELISA at 1:75000. CDK7 antibody can be used for detection of CDK7 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 – 100,000.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight: 39 kDa
Immunogen: Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human CDK7.
Host Species: Rabbit
Purification: Antibody is purified by Protein A/G affinity chromatography method.
Physical State: Lyophilized
CAS NO.: 53-84-9
Product: NAD+
Buffer: Antibody is lyophilized in PBS buffer with 2% sucrose. Add 100 μL of distilled water. Final antibody concentration is 1 mg/mL.
Concentration: 1 mg/ml
Storage Conditions: For short periods of storage (days) store at 4˚C. For longer periods of storage, store CDK7 antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: CDK7, CAK1, HCAK, MO15, STK1, CDKN7, p39MO15
Accession NO.: NP_001790
Protein Ino: 4502743
Official Symbol: CDK7
Geneid: 1022
Background: Cdk7 is the catalytic subunit of the CDK-activating kinase (CAK) complex, a serine-threonine kinase. CAK activates the cyclin-associated kinases CDC2/CDK1, CDK2, CDK4 and CDK6 by threonine phosphorylation. CAK complexed to the core-TFIIH basal transcription factor activates RNA polymerase II by serine phosphorylation of the repetitive carboxyl-terminus domain (CTD) of its large subunit (POLR2A), allowing its escape from the promoter and elongation of the transcripts. Cdk7 is involved in cell cycle control and in RNA transcription by RNA polymerase II. Its expression and activity are constant throughout the cell cycle.
PubMed ID:http://aac.asm.org/content/35/9/1729.abstract
