Product Name: BTK Antibody [7F12H4]
Species Reactivity: Human
Tested Applications: ELISA, IHC, WB
Applications: Western Blot:1:500 – 1:2,000 IHC(P):1:500 – 1:2,000ELISA:Propose dilution 1:10,000.Determining optimal working dilutions by titration test.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Ni-NTA purified truncated recombinant BTK expressed in E. Coli strain BL21 (DE3).
Host Species: Mouse
Purification:
Physical State:
CAS NO.: 1227-61-8
Product: Mefexamide
Buffer: Ascitic fluid containing 0.03% sodium azide.
Concentration:
Storage Conditions: BTK monoclonal antibody can be stored at -20˚C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Monoclonal
Conjugate: Unconjugated
Alternate Names: AGMX1, ATK, BPK, Agammaglobulinemia tyrosine kinase, AT, XLA, IMD1, AGMX1, PSCTK1
Accession NO.: Q06187
Protein Ino: 547759
Official Symbol: BTK
Geneid: 695
Background: Brutons tyrosine kinase (BTK) is a member of the BTK/Tec family of cytoplasmic tyrosine kinases.All members of the family contain SH3 and SH2 domains and, with the exception of Txk and Dsrc28C, also contain a pleckstrin homology (PH) and a Tec homology (TH) domain in their amino termini.BTK plays an important role in B cell development. Activation of B cells by various ligands is accompanied by BTK membrane translocation mediated by its PH domain binding to phosphatidylinositol-3,4,5-trisphosphate. The membrane located BTK is active and associated with transient phosphorylation of two tyrosine residues, Tyr551 and Tyr223. Tyr551 in the activation loop is transphosphorylated by the Src family tyrosine kinase, leading to autophosphorylation at Tyr223 within the SH3 domain, which is necessary for full activation.
PubMed ID:http://aac.asm.org/content/42/6/1515.abstract
