Product Name: ARNT Antibody
Species Reactivity: Human
Tested Applications: ELISA, IHC
Applications: ARNT antibody can be used in ELISA starting at 1:20000 – 1:100000, Western Blot starting at 1:500 – 1:5000, immunohistochemistry starting at 5 μg/mL, and immunofluorescence.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: Amino acids 528 to 544 of human ARNT
Host Species: Rabbit
Purification: Protein G Column
Physical State: Liquid
CAS NO.: 117570-53-3
Product: DMXAA
Buffer: PBS, 0.1% sodium azide.
Concentration:
Storage Conditions: Antibody should be stored long term (months) at -20 ˚C and short term (weeks) at 4 ˚C. As with all antibodies avoid freeze/thaw cycles.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ARNT, ARNT protein, BHLHe2, HIF-1beta, HIF1-beta, HIF1BETA, TANGO, HIF-1-beta, HIF1B
Accession NO.: P27540
Protein Ino: 114163
Official Symbol: ARNT
Geneid: 405
Background: Transcriptional responses to many environmental and developmental stimuli are mediated by the basic helix-loop-helix (bHLH)-PAS family of proteins, which form homodimers that bind regulatory DNA sequences. ARNT/HIF-1 beta regulates the expression of different sets of genes in response to distinct environmental challenges. ARNT forms a complex with arylhydrocarbon receptor (AHR) to activate expression of genes encoding P450. Once AHR binds it ligand, it associates with ARNT for transport into the nucleus. ARNT also dimerizes with HIF-1alpha, -2 alpha or -3 alpha in response to low oxygen concentration to induce the expression of hypoxia-regulated genes encoding vascular endothelial growth and other genes. The expression of ARNT/HIF-1beta remains fairly constant and the protein is found in the nucleus and the cytoplasm.
PubMed ID:http://aac.asm.org/content/53/5/2129.abstract
