Product Name: ABCC4 Antibody
Species Reactivity: Human, Rat
Tested Applications: ELISA, ICC, IF, IHC-P, WB
Applications: ELISA: antibody detection limit dilution 1:32000. Western Blot: Approx 170kDa band observed in rat renal cortex membranes (calculated MW of 149kDa according to Rat NP_596902.1 and 150kDa according to Human NP_005836.2). Data obtained from a previous batch and kindly provided by Dr. Antje Steinbach, Er Immunohistochemistry: In paraffin embedded Human Prostate shows strong cytoplasmic staining in small flat basal cells and weak cytoplasmic staining in the columnar secretory cells of the epithelium. Recommended concentration, 2-4μg/ml. Immunocytochemistry/Immunofluorescence: This antibody (from a previous batch) has been successfully used in ICC/IF for human primary hepatocytes as described in the following paper: Xu et al, Am J Physiol Gastrointest Liver Physiol. 2010
User Note:
Predicted Molecular Weight:
Immunogen: ABCC4 antibody was raised against a 15 amino acid synthetic peptide near the C-Terminus of ABCC4.
Host Species: Goat
Purification: ABCC4 antibody was purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Physical State: Liquid
CAS NO.: 875051-72-2
Product: PF-01247324
Buffer: ABCC4 antibody is supplied in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin.
Concentration: 500 ug/mL
Storage Conditions: Aliquot and store at -20˚C. Minimize freezing and thawing.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: ABCC4, MRP4, MOATB, MOAT-B, EST170205, ATP-binding cassette, sub-family C (CFTR/MRP), member 4, canalicular multispecific organic anion transporter (ABC superfamily), Multidrug Resistance Protein 4
Accession NO.: NP_005836.2
Protein Ino: 157502201
Official Symbol: ABCC4
Geneid: 10257
Background:
PubMed ID:http://aac.asm.org/content/51/12/4462.abstract
