Product Name: 4-1BBL Antibody (biotin)
Species Reactivity: Human
Tested Applications: ELISA, WB
Applications: ELISA:Direct:To detect h4-1BBL by direct ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with compatible secondary reagents, allows the detection of at least 0.2 – 0.4 ng/well of recombinant h4-1BBL.SandwichTo detect h4-1BBL by sandwich ELISA (using 100 μL/well antibody solution) a concentration of 0.25 – 1.0 μg/mL of this antibody is required. This biotinylated polyclonal antibody, in conjunction with our polyclonal Anti-Human 4-1BBL as a capture antibody, allows the detection of at least 0.2 – 0.4 ng/well of recombinant h4-1BBL. Western Blot:To detect h4-1BBL by Western Blot analysis this antibody can be used at a concentration of 0.1 – 0.2 μg/mL. Used in conjunction with compatible secondary reagents the detection limit for recombinant h4-1BBL is 1.5 – 3.0 ng/lane, under either reducing or non-reducing conditions.
User Note: Centrifuge vial prior to opening.
Predicted Molecular Weight:
Immunogen: Produced from sera of rabbits pre-immunized with highly pure (>98%) recombinant h4-1BBL (human 4-1BBLigand). Human 4-1BBL specific antibody was purified by affinity chromatography and then biotinylated.
Host Species: Rabbit
Purification:
Physical State: Lyophilized
CAS NO.: 16941-32-5
Product: Glucagon
Buffer:
Concentration:
Storage Conditions: 4-1BBL antibody is stable for at least 2 years from date of receipt at -20˚C. The reconstituted antibody is stable for at least two weeks at 2-8˚C. Frozen aliquots are stable for at least 6 months when stored at -20˚C. Avoid repeated freeze-thaw cycles.
Clonality: Polyclonal
Conjugate: Biotin
Alternate Names: CD137L, 4-1BB-LTumor necrosis factor ligand superfamily member 9, 4-1BB ligand, 4-1BBL
Accession NO.: P41273
Protein Ino: 728739
Official Symbol: TNFSF9
Geneid: 8744
Background:
PubMed ID:http://aac.asm.org/content/51/11/3830.abstract
