Product Name: 4E-BP1 Antibody
Species Reactivity: Human, Mouse
Tested Applications: ELISA, ICC, IF, WB
Applications: 4E-BP1 antibody can be used for the detection of 4E-BP1 by Western blot at 2.5 – 10 μg/mL. Antibody can also be used for immunocytochemistry starting at 2 μg/mL. For immunofluorescence start at 20 μg/mL.
User Note: Optimal dilutions for each application to be determined by the researcher.
Predicted Molecular Weight:
Immunogen: 4E-BP1 antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human 4E-BP1.The immunogen is located within the last 50 amino acids of 4E-BP1.
Host Species: Rabbit
Purification: 4E-BP1 Antibody is affinity chromatography purified via peptide column.
Physical State: Liquid
CAS NO.: 1673534-76-3
Product: PD-1-IN-1
Buffer: 4E-BP1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration: 1 mg/mL
Storage Conditions: 4E-BP1 antibody can be stored at 4˚C for three months and -20˚C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Clonality: Polyclonal
Conjugate: Unconjugated
Alternate Names: 4E-BP1 Antibody: BP-1, 4EBP1, 4E-BP1, PHAS-I, Eukaryotic translation initiation factor 4E-binding protein 1, Phosphorylated heat- and acid-stable protein regulated by insulin 1
Accession NO.: NP_004086
Protein Ino: 4758258
Official Symbol: EIF4EBP1
Geneid: 1978
Background: 4E-BP1 Antibody: The translation of mRNA in eukaryotic cells is regulated by the presence of amino acids through multiple mechanisms. One such mechanism involves the evolutionarily conserved serine/threonine kinase TOR (Target of rapamycin, also known as mTOR), which regulates cell growth and cell cycle through its ability to integrate signals from nutrient levels and growth factors. One downstream target of TOR is the eukaryotic initiation factor 4E binding protein 1 (4E-BP1) whose phosphorylation prevents its association with eIF4E, preferentially stimulating translation of mRNAs containing long, highly structured 5-UTRs. Rapamycin inhibits TOR resulting in reduced cell growth and reduced rates of cell cycle and cell proliferationl, at least in part by inhibiting the activity of TOR towards 4E-BP1.
PubMed ID:http://aac.asm.org/content/51/11/3836.abstract
